The effect of a novel botanical agent on early osteogenic response of osteoblasts
선영곤, 김영태, Piao Xing-Hui, 문종욱, 김영준,
소속 상세정보
선영곤 ( Sun Young-Gon ) - Chonnam National University School of Dentistry Department of Periodontology
김영태 ( Kim Young-Tae ) - Chonnam National University School of Dentistry Department of Periodontology
( Piao Xing-Hui ) - Chonnam National University School of Dentistry Department of Periodontology
문종욱 ( Moon Jong-Wook ) - Chonnam National University School of Dentistry Department of Periodontology
김영준 ( Kim Young-Joon ) - Chonnam National University School of Dentistry Department of Periodontology
Abstract
A mixture of Panax notoginseng and Rehmannia glutinosa libosch (BT-301) is a synthetically developed novel botanical agent having anti-inflammatory properties. Previous studies have shown that BT-301 prevents the destruction of periodontal tissue by inhibiting the secretion of several cytokines (TNF-α, IL-6). It was also reported that Panax notoginseng and Rehmannia glutinosa libosch have a positive influence on osteoblastic cells. However, related literatures on Panax notoginseng and Rehmannia glutinosa libosch are rare. This study aimed to evaluate the cell proliferation and osteogenic effects of BT-301 on osteoblastic cells. In dose and time kinetic studies performed, MC3T3-E1 cells cultured in α-MEM medium were treated with varying concentrations of BT-301 (control, 0.156, 0.312, 0.625, 1.25, 2.5 mg/ml), and incubated for 1, 3, and 5 days. Cell proliferation was assessed by the MTT assay. Alkaline phosphatase (ALP) activity of MC3T3-E1 cells was measured on day 7. Results show that MC3T3-E1 cells exposed to BT-301 at 0.156 mg/ml and 0.312 mg/ml concentrations, showed greater cell proliferation on day 3 (p<0.05). We conclude that correct exposure to the optimal concentration of BT-301 has a positive effect on bone healing.
키워드
Alkaline phosphatase (ALP); Cell proliferation; Panax notoginseng; Rehmannia glutinosa libosch
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